BIM-800FLW Inverted Epi-Fluorescent Biological Microscope

Introduction BIM-800FLW Inverted Fluorescent Biological Microscope is specifically designed for the observation of cell culture with high quality optics and advanced fluorescent imagig capability. Infinity correcetd optical system gives excellent imaging performance. Super high resolution fluorescent objectives are offered to generate high quality fluorescent images. This user-friendly microscope acquisition systems allows undergraduate and graduate students with various degrees of microscopy experience to examine a wide range of samples  such as live cells, fixed cell, histological preps, microfluidic devices, bead samples, range of fluorophores, etc with high reproducibility. Overview UCIS Infinity Independent Achromatic Optical System WF10x plan eyepieces, 20mm field of view, high eye point up to 21mm Seidentopf binocular viewing head; inclined45º 52-75mm interpupillary distance settings ±5 Diopter adjustment Quintuple nosepiece inward facing with positive click stops Infinity long work distance plan Achromatic 10x, 20x, 40x and Infinity long work distance phase-contrast 20x objectives Right hand controls, low position, fixed stage, 240mm x 260mm, movement range 135mm x 85mm N.A. 0.30 Abbe condenser with iris diaphragm, with phase-contrast slide, long work distance 72mm. Coaxial coarse and fine focus mechanism with markings. Fine focus sensitivity 0.001mm 6 volt 30 watt halogen illumination Fluorescence illumination system: 100W mercury lamphouse and power supply, B/G/UV/V fluorescence filter block Dust cover, power cord Applications It can be used to observe transparent livings which are not stained, particularly living cells, tissues, fluids, sediments etc. It is an ideal instrument for biology, cytology, oncology, genetics, immunology and other research works. It can be used in scientific research institutes, universities, medical care centers, disease prevention, quality inspection, agriculture, animal husbandry and other departments. It can also be used in venereal disease examination and immune diagnosis. 1. Main Body 2. Optical System Ask us how to add or swap with Japanese objective lenses and save lots of money (1,25x, 2.5x, 4x, 10x, 20x, 40x (dry or oil), 50x(dry or oil), 60x (dry), 100x (oil)). Bright Field & Fluor Objective Lenses Phase Contrast Objectives and Slider 3. Mechanical XY Stage Attachable Mechanical Stage, X-Y Coaxial Control，Moving Rang120mm×78mm (Optional)  Ruler to show a well location in the 96-well plates   35mm Culture Dish Holder 54mm Culture Dish Holder & Glass slide holder 65mm Culture Dish Holder & Terasaki Holder 4. Video Imaging Easy instal a camera from the side video port switch between eyepieces & camera (0/100%), perfect for epi-fluor application Instal a side tablet camera for professional observation 5. Plenty of Accessories 6. Epi-Fluorescent Compnents: a) Filter Wheel with 5 spots    b) Filter  Sets: B, G, UV, V Excitation filter B/G/U/V, CHROMA Brand Blue Excitation Filter, BP460~490, DM500, BA520 Green Excitation Filter, BP510~550, DM570, BA590 Ultraviolet Excitation Filter, BP330~385, DM400, BA420 Violet Excitation Filter, BP400~410, DM455, BA455   Ask us how to add a new filter set with any specifiction you want: c) illumination SAMSUNG CSC[/caption] 100W Mercury Lamp-House HBO Power Source Fluor alignment obj lens (optional, recommended)  GFP  RFP 7. System Diagram 8. Specification Specification BIM800FLW   Inverted Fluorescent Microscope 2 4 Optical System UCIS infinity independent achromatic optical system Frame One-piece construction from arm to base Head Seidentopf binocular head, 45° inclined, interpupillary distance 52-75mm, ±5 diopter adjustment Eyepiece WF10x/20mm, plan, high eyepoint up to 21mm, Centering Attachment Centering telescope Objective Infinity LWD plan achromatic objective 10x/0.25  WD9.67 Infinity LWD plan achromatic objective 20x/0.40  WD7.97, Infinity LWD plan achromatic objective 40x/0.60  WD3.76 Infinity LWD plan phase contrast objective 20x/0.40  WD7.97 Focus System Low positioned, coaxial coarse & fine focusing system with adjustable tension control and upper limit stop, minimum reading 1μm, moving range 12mm Nosepiece Quintuple, reversed type with positive click stops Condenser Abbe condenserN.A. 0.30 with iris diaphragm, with phase contrast slider & filter (φ34) blue,green & yellow, long work distance 72mm Stage Right hand controls, low position, mechanical stage, size 240x260mm, moving range 135x85mm. Drop specimen holder(φ118) Specimen holder (76×26, φ54) Light Source 6V30W Halogen lamp house Electrical part for 6V30W halogen lmap 6V30W halogen bulb (Osram) 250V/3.15A Φ5X20 Fuse Power supply 110-240V，50/60Hz Fluorescence 100W DC Mercury lamp house Power supply unit for 100W DC mercury lamp Epi-fluorescent illumination,multi-functional semi-reflector 100W Mercury bulb (Osram) 250V/5A Φ5X20 Fuse Excitation filter B/G, CHROMA Brand —Blue Excitation Filter, BP460~490, DM500, BA520 —Green Excitation Filter, BP510~550, DM570, BA590 X Excitation filter B/G/U/V, CHROMA Brand —Blue Excitation Filter, BP460~490, DM500, BA520 —Green Excitation Filter, BP510~550, DM570, BA590 –Ultraviolet Excitation Filter, BP330~385, DM400, BA420 –Violet Excitation Filter, BP400~410, DM455, BA455 X Phase Contrast Phase contrast slider for 10x,20x,40x Adapter 0.5x, C-Mount Other Allen screw driver 2mm Allen screw driver 2.5mm Dust cover Power cord Optional Accessories Item No. Head Seidentopf binocular head, 45° inclined, interpupillary distance 48-75mm, diopter adjustable BIM800FLW-BH Eyepiece WF10x/22mm plan eyepiece, high eyepoint BIM800FLW-10×22 WF10x/20mm, plan, high eyepoint up to 21mm, diopter adjustable BIM800FLW-10×20 WF16x/13mm, plan BIM800FLW-16×13 Objective Infinity plan achromatic objective 4x BIM800FLW-4xbf Infinity LWD plan achromatic objective 60x BIM800FLW-60xLWD Infinity LWD plan phase-contrast objective 10x (positive) BIM800FLW-10xPB Infinity LWD plan phase-contrast objective 40x (positive) BIM800FLW-40xPB Infinity LWD Plan Phase-Contrast Objective 10x (Negative) BIM800FLW10xNB Infinity LWD Plan Phase-Contrast Objective 20x (Negative) BIM800FLW-20xNB Infinity LWD Plan Phase-Contrast Objective 40x (Negative) BIM800FLW-40xNB Stage Mechanical Stage, Size 350x208mm，Moving Range 50x50mm BIM800FLW-xy50 Adapter 0.25x C-Mount BIM800FLW-0.25XC Digital camera adapter (need to know camera brand) BIM800FLW-DSLR-X Bulb 6V30W Halogen bulb BIM800FLW-6v30W Specimen Holder 125×85 BIM800FLW-XY-125 Fluorescence Attachment 100W DC Mercury lamp house BIM800FLW-HBO Power supply unit for 100W DC mercury lamp BIM800FLW-HBO-PS Epi-fluorescent illumination,multi-functional semi-reflector BIM800FLW-RF Excitation Filter B, CHROMA Brand BIM800FLW-IB Excitation Filter G, CHROMA Brand BIM800FLW-IG Excitation Filter U, CHROMA Brand BIM800FLW-IUV Excitation Filter V, CHROMA Brand BIM800FLW-IV 100W Mercury bulb BIM800FLW-HBO-C 100W Mercury bulb (Osram) BIM800FLW-HBO-O Empty Filter Cube BIM800FLW-F Sample Images 10x Ph-postive lens, BF mode, 0.5x c-mount adapter 20x Ph-positive lens, BF mode, 0.5x c-mount adapter 40x Ph-positive lens, BF mode, 1x c-mount adapter 60x Ph-positive lens, BF mode, 1x c-mount adapter 10x Ph-positive lens, Ph mode, 0.5x c-mount adapter 20x Ph-positive lens, Ph mode, 0.5x c-mount adapter 40x Ph-positive lens, Ph mode, 1x c-mount adapter  40x Ph-negative lens, Ph mode, 1x c-mount adapter Following images were collected using this microscope, BIC-C30 3MP sCMOS Camera, BIM800-0.5x 0.5x C-mount adapter and the below-mentioned objective lenses. merged images were generated using Image software. FluoCells® Prepared Slide #2: bovine pulmonary artery endothelial cells (BPAE) cells with Mouse Anti-α-tubulin, BODIPY FL Goat Anti-Mouse IgG, Texas Red-X Phalloidin, and DAPI), (Molecular Probes) Obj Lens UV Excitation Blue Excitation Green Excitation Merged / Superimposed 2-3 Channels 20x BIHR-20xBF 40x BIMJ834-40x 50x LWD BIHR-50xBFL 60x BIMJ969-60x  FluoCells® Prepared Slide #6 (Muntjac cells with Mouse Anti-OxPhos Complex V Inhibitor Protein, Alexa Fluor 555 Goat Anti-Mouse IgG, Alexa Fluor 488 Phalloidin, and TO-PRO-3) (Molecular Probes) 5x BIHR-5xBF 40x BIMJ834-40x 60x BIMJ969-60x  20x BIHR-20xBF 20x BIHR-20xBF 40x BIMJ834-40x