Plan Fluor Objective lenses

Epi-Fluorescent Objective lenses  Apochromat lenses are the highest quality and have been corrected to bring 3 wavelengths (red, green & blue) into focus on the same plane in epi-fluorescent microscopes such as IncuScope. Apochromat lenses have a flat field of view, meaning that the curvature of the lens has been corrected so that the entire circular image is crisp and in focus. Plan Achromat Lenses are the mid-range quality lens and have been corrected to bring 2 wavelengths (red & blue) into focus on the same plane. Plan lenses also have a flat field of view. Achromat lenses are the basic quality lens and have been corrected to bring 2 wavelengths (red & blue) into focus on the same plane. However, without the “plan” indication – these lenses have not been corrected for the curvature of the lens and do not have a completely flat field of view – meaning the very outer edges of the circular image viewed will be slightly out of focus due to that curvature of the lens. Lens Type Quality Corrected for Field of View Apochromat Best 3 Wavelengths Flat Plan Achromat Middle 2 Wavelengths Flat Achromat Lowest 2 Wavelengths Not Flat High NA Plan Fluor Lenses Magnification NA WD (mm) F’ (mm) R (μ)   DF (μ) FOV On object (mm) FOV on image (mm) Thread 10x 0.40 3.6 20.0 0.74 1.65 2.2 22.0  RMS 20x 0.75 0.6 10.0 0.45 0.59 1.1 22.0  RMS 40x 0.95 0.17 5.0 0.35 0.37 0.55 22.0  RMS 100x (water) 1.20 0.16 2.0 0.28 0.23 0.22 22.0  RMS 100x (Oil) 1.35 0.08 2.0 0.25 0.18 0.22 22.0  RMS RMS = 0.800″-36